마우스 골수세포 배양시 transforming growth factor-β와 epidermal growth factor 가 파골세포양세포의 형성에 미치는 영향
Effects of Transforming Growth Factor-β and Epidermal Growth Factor on the Osteoclast-like Cell Formation in the Mouse Bone Marrow Cell Culture.
소속 상세정보
1임충남/1Choong Nam Lim
1김세원/2고선일/2김정근/1Se Won Kim/2Seon Yle Ko/2Jung Keun Kim
KMID : 0355220000250010053
Abstract
Bone marrow culture systems are widely used to differentiate osteoclast-like cells
invitro using several osteotropic hormones. In this study, we isolated and cultured the
mouse bone marrow cells with or without some osteotropic hormones such as
parathyroid hormone(PTH), prostaglandin E2(PGE2) and
1,25(OH)2-vitamin D3(Vit. D2). We confirmed
the formation of osteoclast-like cells morphologically and functionally by the expression
of tartrate-resistant acid phosphatase(TRAP) and by their capability to resorb dentin
slices. We also studied the effects of transforming growth factor-β(TGF-β)and
epidermal growth factor(EGF) on the Vit. D3-induced osteoclast-like cell
formation. In control, a few multinucleated cells were formed whereas PTH and
PGE2 increased the number of multinucleated cells. PTH,
PGE2 and Vit. D3 induced the formation of TRAP-positive
multinucleated cells. After culture of mouse bone marrow cells on the dentin slices with
or without osteotropic hormones, giant cells with diverse morphology were found on the
dentin slice under the scanning electronmicroscopy. After removing the attached cells,
resorption pits were identified on the dentin slices, and the shape of resorption pit were
identified on the dentin slices, and the shape of resorption pits was variable. EGF
increased the osteoclast-like cell formation induced by Vit. D3, however,
TFG-β showed biphasic effect, which at low concentration, increased and at high
concentration, decreased the osteoclast-like cell formation induced by Vit.
D3.
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